Part:BBa_K1199005:Design

LinB 1,3,4,6-tetrachloro-1,4-cyclohexadiene->2,5-dichloro-2,5-cyclohexadiene-1,4-diol

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 157
  Illegal NgoMIV site found at 648
  Illegal NgoMIV site found at 682
  Illegal AgeI site found at 82
  Illegal AgeI site found at 748
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Make sure your sequence must not contain the restriction enzyme sites

Source

LinB is cofactor-independent dehalogenase from Pseudomonas paucimobilis UT26